Hek cells

HEK-Zellen ist die abgekürzte Bezeichnung für Human Embryonic Kidney-Zellen, menschliche embryonale Nierenzellen (HEK). Diese Zelllinie wird auch als HEK-2oder 293-Zellen bezeichnet. HEK-Zellen werden in der Zellbiologie seit vielen Jahren als vergleichsweise einfach zu handhabende Zelllinie eingesetzt.

HEK 29 and especially HEK 293T, cells are commonly used for the production of various retroviral vectors. Various retroviral packaging cell lines are also based on these cells. Native proteins of interest. Depending on various conditions, the gene expression of HEK 2cells may vary. The following proteins of interest.

Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. L of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. HEK 2cells are popular for their ease of growth and transfection (HEK2Transfection Kit), making them a common cell culture in cancer research.

HEK -2cells are useful for many transfection. In our experience HEK cells containing the EBNA gen allow higher transient transfection efficiencies than 293T cells. Some literature also indicate that the EBNA gen enhances the transport of. HEK 2cells are efficiently transfected with LipofectAMINE, with typically of cells showing fluorescence following transfection with green fluorescent protein (GFP). This is necessary because serum is replete with RNases that degrade ribozymes.

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. In this event, centrifuge contents of flask and reseed to allow re-attachment of cells. These cells should be frozen in DMSO : FCS. They can be used for the screening of anti-IL-antibodies or for IL.

Das folgende Bild zeigt die am häufigsten verwendeten Bedeutungen von HEK. Sie können die Bilddatei im PNG-Format für die Offline-Verwendung herunterladen oder per E-Mail an Ihre Freunde senden. Wenn Sie ein Webmaster einer nichtkommerziellen Website sin können Sie das Bild von HEK-Definitionen auf Ihrer Website veröffentlichen. HEK2are rounded cells that grow in suspension in cell culture, although initially they were an adherent cell line. Subculture Protocol for.

Author information: (1)Department of Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK. The HEK cell line has been extensively used as an expression tool for recombinant proteins since it was generated over years ago. HEK2cell line: a vehicle for the expression of recombinant proteins.

DNA:CaCl mix is added drop-wise over the plated cells and the dishes are returned to the incubator (37°C, CO 2: air) without any disturbance of the dishes. DMEM-based growth medium. The cell line does not adhere to the substrate when left at room temperature for any length of time. The cells will reattach to the flask over a period of several days in culture at 37°C. The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-subunit and the vitronectin receptor alpha-v subunit.

Thawing Cells Use the following procedure to thaw 293FT cells to initiate cell culture. Thaw cells in prewarme complete medium without Geneticin. Remove the vial of frozen cells from liquid nitrogen and thaw quickly in a 37°C water bath.

HEK 2cell, the Human Embryonic Kidney 2cells was originally derived from human embryonic kidney cells grown in tissue culture. Presence of the SVlarge T antigen allows very high levels of protein to be expressed from vectors containing the SVorigin. Higher, in fact, than other 2cells or the T antigen-transformed COS cells classically used.

Die ursprüngliche HEK -Zelllinie ist mit HeLa-Zellen kontaminiert und wird nicht mehr verwendet. Harald zur Hausen: Induction of specific chromosomal aberrations by Adenovirus Typ in human embryonic kidney cells. HEK -Zellen ist die abgekürzte Bezeichnung für Human Embryonic Kidney-Zellen, menschliche embryonale Nierenzellen ( HEK ). HEK -Zellen werden in der Zellbiologie seit vielen Jahren als vergleichsweise einfach zu handhabende Zelllinie eingesetzt.